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Starch, however, cannot be fermented directly by S. cerevisiae, because the organism lacks the requisite starch-degrading or amylolytic enzymes to liberate glucose from this storage polysaccharide. F. Power GLUCOSE ATP Glucokinase ADP Glucose-6-phosphate Phosphohexose isomerase Fructose-6-P ATP Phosphofructokinase ADP Fructose 1,6 bisphosphate Aldolase Glycolysis Dihydroxyacetone phosphate Glyceraldehyde 3-phosphate Triosephosphate isomerase NAD+ Glyceraldehyde-3-P dehydrogenase NADH 1,3 bisphosphoglycerate ADP Phosphoglycerate kinase ATP 3-phosphoglycerate Phosphoglycerate mutase 2-phosphoglycerate Enolase H2O Phosphoenolpyruvate ADP Pyruvate kinase ATP Pyruvate Anaerobic homolactic fermentation Lactic bacteria Lactic acid Aerobic oxidation TCA cycle CO2 + Water Anaerobic alcoholic fermentation Yeast CO2 + Ethanol Figure 1.
Dry milling operations should also incorporate explosion dampers in the system. This brings us to the question of what type of a mill to use. J. Radzanowski Table 1. Recommended malt sieve analysis for lauter tun and mash filter wort separation systems. 0059) Bottom For mash filter (% retained) 14 20 29 18 9 4 6 6 10 6 18 43 10 7 Adapted from ASBC, 1996 with five or six rolls. The roller mills work extremely well with the soft, already modified and malted barley, which is the principal substrate of these operations.
Recent experience, however, shows that DE, provided it is above 10, is of no concern. , lower viscosity, so that mash can be pumped through the heat exchanger to the fermentor (for SSF) or to the saccharification tank. The functional characteristics of liquid glucoamylase prepared from the microorganism Aspergillus niger, can be seen in Figures 15 and 16. Two parameters, temperature and pH, dictate how enzymes can be used. 5 and a temperature of 90°C, this is not at all acceptable for saccharification.